Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p229 | (1) | WCRB2014

Trehalose in glycerol-free freezing extender preserves mitochondria membrane potential of post-thaw boar spermatozoa

Athurupana Rukmali , Funahashi H

Introduction: Movement of spermatozoa and the penetration of zona-pellucida depend on the energy produced in mitochondria. Major steps of cryopreservation can exert stress on sperm mitochondria membrane. Better post-thaw motility, viability and acrosome integrity of spermatozoa was observed when extended in trehalose in a previous study. Objective was to evaluate the effect of trehalose on mitochondria integrity of post-thawed boar spermatozoa.Materials ...

ra0001p123 | (1) | WCRB2014

Relative transcript abundance in porcine cumulus cells collected from different size follicles

Nicolas Carla Moros , Rico Maria Jose Izquierdo , Li Y , Romar Rakel , Funahashi H

Introduction: Bi-directional communication between the oocyte and cumulus cells (CCs) is essential for the production of competent oocytes. Previous studies analyzed the relative transcript abundance in oocytes from small follicles (SF) and medium follicles (MF) to determinate the likely use of oocytes from SF in artificial reproductive techniques. The aim of this study was to examine the relative transcript abundance of CCs from cumulus–oocyte complexes (COCs) from SF an...